A Sensitive and Specific Indirect Competitive Enzyme-Linked Immunosorbent Assay for Detection of Paeoniflorin and Its Application in Pharmacokinetic Interactions between Paeoniflorin and Glycyrrhizinic Acid

作者：Zhao, Yan; Qu, Huihua; Wang, Xueqian; Zhang, Yue; Shan, Wenchao; Zhao, Yan; Wang, Qingguo

来源：Planta Medica, 2015, 81(9): 765-770.

DOI：10.1055/s-0035-1545913

• paeoniflorin
• monoclonal antibody
• enzyme-linked immunosorbent assay (ELISA)
• pharmacokinetics
• glycyrrhizic acid

This study aimed to develop an indirect competitive enzyme-linked immunosorbent assay based on monoclonal antibodies against paeoniflorin to study the effects of different doses of glycyrrhizinic acid on the pharmacokinetics of paeoniflorin in mice. An anti-paeoniflorin monoclonal antibody was produced from a hybridoma created through a fusion of splenocytes immunized with paeoniflorin-bovine serum albumin and conjugated with the hypoxanthine-aminopterin-thymidine-sensitive mouse myeloma cell line SP2/0. The resultant antibody was used to develop and validate a rapid, specific and sensitive, indirect competitive enzyme-linked immunosorbent assay for the measurement of paeoniflorin (linear range 4.8-312.5 ng . mL(-1)). The intraday and interday precision values of the indirect competitive ELISA method were well within the recommended range (<= 10%), and the recovery rate was, on average, 101.13%. Pharmacokinetic parameters obtained from mouse blood samples at various intervals following the oral administration of paeoniflorin and glycyrrhizic acid at three doses (1 : 0.3, 1 : 1, 1 : 3, respectively) demonstrated that the highest dose of glycyrrhizic acid inhibits the absorption of paeoniflorin.

• 出版日期2015-6
• 单位北京中医药大学