A highly sensitive electrochemical biosensor for phenol derivatives using a graphene oxide-modified tyrosinase electrode

作者：Wang, Yue^*; Zhai, Fengge; Hasebe, Yasushi; Jia, Hongmin; Zhang, Zhiqiang^*

来源：Bioelectrochemistry, 2018, 122: 174-182.

DOI：10.1016/j.bioelechem.2018.04.003

• Tyrosinase
• Graphene oxide
• Covalent bonding
• Phenol derivatives
• Glassy carbon electrode

The fabrication, characterization and analytical performance were investigated for a phenol biosensor based on the covalent bonding of tyrosinase (TYR) onto a graphene oxide (GO)-modified glassy carbon electrode (GCE) via glutaraldehyde (GA). The surface morphology of the modified electrode was studied by atomic force microscope (AFM) and field-emission scanning electron microscopy (FE-SEM). The fabricated TYR/GA/GO/GCE biosensor showed very good stability, reproducibility, sensitivity and practical usage. The catechol biosensor exhibited a wide sensing linear range from 5 x 10(-8) M to 5 x 10(-5) M, a lower detection limit of 3 x 10(-8) M, a current maximum (I-max) of 65.8 mu A and an apparent Michaelis constant (K-m(app)) of 169.9 mu M.

• 出版日期2018-8
• 单位辽宁科技大学