A Selective PMCA Inhibitor Does Not Prolong the Electroolfactogram in Mouse

作者：Griff Edwin R^*; Kleene Nancy K; Kleene Steven J

来源：PLos One, 2012, 7(5): e37148.

DOI：10.1371/journal.pone.0037148

Background: Within the cilia of vertebrate olfactory receptor neurons, Ca2+ accumulates during odor transduction. Termination of the odor response requires removal of this Ca2+, and prior evidence suggests that both Na+/Ca2+ exchange and plasma membrane Ca2+-ATPase (PMCA) contribute to this removal. %26lt;br%26gt;Principal Findings: In intact mouse olfactory epithelium, we measured the time course of termination of the odor-induced field potential. Replacement of mucosal Na+ with Li+, which reduces the ability of Na+/Ca2+ exchange to expel Ca2+, prolonged the termination as expected. However, treating the epithelium with the specific PMCA inhibitor caloxin 1b1 caused no significant increase in the time course of response termination. %26lt;br%26gt;Conclusions: Under these experimental conditions, PMCA does not contribute detectably to the termination of the odor response.

• 出版日期2012-5-15