Activity of an enzyme immobilized on superparamagnetic particles in a rotational magnetic field

作者：Mizuki Toru; Watanabe Noriyuki; Nagaoka Yutaka; Fukushima Tadamasa; Morimoto Hisao; Usami Ron; Maekawa Toru^*

来源：Biochemical and Biophysical Research Communications, 2010, 393(4): 779-782.

DOI：10.1016/j.bbrc.2010.02.081

• Enzyme
• Activity
• Immobilization
• Nanoparticles
• Magnetic particles
• Magnetic field

We immobilize alpha-amylase extracted from Bacillus licheniformis on the surfaces of superparamagnetic particles and investigate the effect of a rotational magnetic field on the enzyme's activity. We find that the activity of the enzyme molecules immobilized on superparamagnetic particles increases in the rotational magnetic field and reaches maximum at a certain frequency. We clarify the effect of the cluster structures formed by the superparamagnetic particles on the activity. Enzyme reactions are enhanced even in a tiny volume of solution using the present method, which is very important for the development of efficient micro reactors and micro total analysis systems (mu-TAS).

• 出版日期2010-3-19