A Transgenic Zebrafish Model of Targeted Oocyte Ablation and de novo Oogenesis

作者：White Yvonne A R^*; Woods Dori C; Wood Antony W

来源：Developmental Dynamics, 2011, 240(8): 1929-1937.

DOI：10.1002/dvdy.22695

• zebrafish
• oogenesis
• transgenics
• ovary
• oocyte
• stem cell
• ablation

We describe here a novel transgenic zebrafish, Tg(zpc:G4VP16/UAS:nfsB-mCherry) that effectively demonstrates the targeted oocyte ablation in the adult zebrafish ovary. This transgenic line expresses bacterial nitroreductase enzyme (nfsB) under the control of the oocyte-specific zona pellucida C (zpc) gene promoter. Adult transgenic females exposed to the prodrug metronidazole demonstrated near-complete ablation of growing oocytes, resulting in ovarian degeneration and complete cessation of reproductive function. Within 4 weeks of prodrug removal, treated fish demonstrated complete anatomical regeneration of the ovary and, within 7 weeks, ovarian fertility) was fully restored. Together, these results demonstrate functional renewal of the oocyte pool in the adult zebrafish ovary. Accordingly, this transgenic zebrafish model system provides a novel means to investigate ovarian growth dynamics in a genetically tractable vertebrate, and may be useful for evaluating signaling interactions that regulate gonadal development processes such as de novo oogenesis. Developmental Dynamics 240:1929-1937, 2011.

• 出版日期2011-8