Anti-proliferative effect of glucocorticoids on mesenchymal cells in juvenile angiofibromas

作者：Wendler Olaf; Dlugaiczyk Julia; Birk Stephanie; Schick Bernhard^*

来源：Head and Neck-Journal for the Sciences and Specialties of the Head and Neck, 2012, 34(11): 1615-1621.

DOI：10.1002/hed.21966

• juvenile angiofibroma
• nasal mucosa
• glucocorticoid receptor
• dexamethasone
• proliferation

Background Glucocorticoids (GCs) not only regulate metabolic and inflammatory mechanisms, but also are known to suppress tumor growth. Despite previous detection of glucocorticoid receptors (GRs) in juvenile angiofibromas, their distribution and function have not further been studied. Methods Juvenile angiofibroma tissue (n = 30), nasal mucosa specimens (n = 10), subepithelial stroma of nasal mucosa (n = 20), and primary fibroblasts from juvenile angiofibroma (n = 6) and nasal mucosa samples (n = 6) were analyzed by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and immunofluorescence staining. The antiproliferative effect of GCs (dexamethasone, prednisolone, and hydrocortisone) in vitro was assessed using a bromdeoxyuridine (BrdU) assay. Results An upregulation of GR transcripts and protein was shown in juvenile angiofibroma tissue and primary mesenchymal cells compared to nasal mucosa. Application of GCs resulted in a significantly higher antiproliferative effect on juvenile angiofibroma versus nasal mucosa fibroblasts in vitro. Conclusion Expression of GRs and antiproliferative effects of GCs on juvenile angiofibroma fibroblasts offer novel options for the treatment of this unique fibrovascular tumor.

• 出版日期2012-11