Assessment of potato proteinase inhibitor-II gene as an antifungal and insecticidal agent

作者：Majeed Asifa^*; Makhdoom Rahat; Husnain Tayyab; Riazuddin Sheikh

来源：Acta Agriculturae Scandinavica Section B-Soil and Plant Science, 2011, 61(1): 92-96.

DOI：10.1080/09064710903433777

• Bt
• Helicoverpa armigera
• pinII
• Rhizoctonia
• tobacco

The aim of this study was to investigate the protection role of potato proteinase inhibitor-II gene against insect and fungal pathogens of cotton. The positive integration of pinII was confirmed by polymerase chain reaction and Southern analysis in transgenic tobacco plants. In insect bioassay, the transgenic lines were more resistant to cotton bollworm (Helicoverpa armigera) and caused insect mortality and severe growth inhibition. Expression against Rhizoctonia implicated a pivotal role of this gene in defence towards pathogens. Potato proteinase inhibitor-II had shown significant expression against insect as well as fungal pathogens and suggests the role of pinII in engineering insect/pathogen-resistant plants.

• 出版日期2011