Ultrafast Structural Dynamics of BlsA, a Photoreceptor from the Pathogenic Bacterium Acinetobacter baumannii

作者：Brust Richard; Haigney Allison; Lukacs Andras; Gil Agnieszka; Hossain Shahrier; Addison Kiri; Lai Cheng Tsung; Towrie Michael; Greetham Gregory M; Clark Ian P; Illarionov Boris; Bacher Adelbert; Kim Ryu Ryun; Fischer Markus; Simmerling Carlos; Meech Stephen R^*; Tonge Peter J

来源：Journal of Physical Chemistry Letters, 2014, 5(1): 220-224.

DOI：10.1021/jz4023738

Acinetobacter baumannii is an important human pathogen that can form biofilms and persist under harsh environmental conditions. Biofilm formation and virulence are modulated by blue light, which is thought to be regulated by a BLUF protein, BlsA. To understand the molecular mechanism of light sensing, we have used steady-state and ultrafast vibrational spectroscopy to compare the photoactivation mechanism of BlsA to the BLUF photosensor AppA from Rhodobacter sphaeroides. Although similar photocycles are observed, vibrational data together with homology modeling identify significant differences in the beta 5 strand in BlsA caused by photoactivation, which are proposed to be directly linked to downstream signaling.

• 出版日期2014-1-16