A paramagnetic implant containing lithium naphthalocyanine microcrystals for high-resolution biological oximetry

作者：Meenakshisundaram Guruguhan; Pandian Ramasamy P; Eteshola Edward; Lee Stephen C; Kuppusamy Periannan^*

来源：Journal of Magnetic Resonance, 2010, 203(1): 185-189.

DOI：10.1016/j.jmr.2009.11.016

• Oxygen sensor
• High-sensitivity probe
• Polymer coating
• Biocompatible EPR probe

Lithium naphthalocyanine (LiNc) is a microcrystalline EPR oximetry probe with high sensitivity to oxygen [R P. Pandian. M Dolgos, C. Marginean, P M Woodward, P C Hammel, P T Manoharan, P Kuppusamy, Molecular packing and magnetic properties of lithium naphthalocyanine crystal hollow channels enabling permeability and paramagnetic sensitivity to molecular oxygen J Mater Chem 19 (2009) 4138-4147] However, direct implantation of the crystals in the tissue for in vivo oxygen measurements may be hindered by concerns associated with their direct contact with the tissue/cells and loss of EPR signal due to particle migration in the tissue In order to address these concerns, we have developed encapsulations (chips) of LiNc microcrystals in polydimethyl siloxane (PDMS), an oxygen-permeable bio-inert polymer Oximetry evaluation of the fabricated chips revealed that the oxygen sensitivity of the crystals was unaffected by encapsulation in PDMS. Chips were stable against sterilization procedures or treatment with common biological oxidoreductants In vivo oxygen measurements established the ability of the chips to provide reliable and repeated measurements Of tissue oxygenation This study establishes PDMS-encapsulated LiNc as a potential probe for long-term and repeated measurements of tissue oxygenation.

• 出版日期2010-3