A new method for non-invasive biomass determination based on stereo photogrammetry

作者：Syngelaki Maria; Hardner Matthias; Oberthuer Patrick; Bley Thomas; Schneider Danilo; Lenk Felix^*

来源：Bioprocess and Biosystems Engineering, 2018, 41(3): 369-380.

DOI：10.1007/s00449-017-1871-2

• Biomass calculation
• Photogrammetry
• Plant cell tissue
• Imaging
• Image analysis

A novel, non-destructive method for the biomass estimation of biological samples on culture dishes was developed. To achieve this, a photogrammetric system, which consists of a digital single-lens reflex camera (DSLR), an illuminated platform where the culture dishes are positioned and an Arduino board which controls the capturing process, was constructed. The camera was mounted on a holder which set the camera at different title angles and the platform rotated, to capture images from different directions. A software, based on stereo photogrammetry, was developed for the three-dimensional (3D) reconstruction of the samples. The proof-of-concept was demonstrated in a series of experiments with plant tissue cultures and specifically with calli cultures of Salvia fruticosa and Ocimum basilicum. For a period of 14 days images of these cultures were acquired and 3D-reconstructions and volumetric data were obtained. The volumetric data correlated well with the experimental measurements and made the calculation of the specific growth rate, A mu (max), possible. The A mu (max) value for S. fruticosa samples was 0.14 day(-1) and for O. basilicum 0.16 day(-1). The developed method demonstrated the high potential of this photogrammetric approach in the biological sciences.

• 出版日期2018-3