科研之友
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摘要
This article investigated the potential of substrate characteristics for the control of differentiation of stem cells. For this, two distinctive materials, alginate and HA (hydroxiapatite) contained PCL, were selected. Utilizing rapid prototype (RP) technique we fabricated each type of scaffold. Mesenchymal stem cells (MSCs) derived from New Zealand White rabbits were suspended into alginate solution and the solution was plotted utilizing RP technique. The cocktail media which contain chondrogenic and osteogenic growth factors were used. The basal media were also used for comparison. During 14 days of experiments the samples were harvested four times. The results showed that MSCs tended to be differentiated into osteo- and chondrocyte-like cells when they were on PCL and in alginate scaffold, respectively even both of the two types of scaffolds were in basal media. These findings were more observable when the cocktail media were used. From this research we can stipulate that the characteristics of substrate materials should be considered along with biochemical reagents in the control of differentiation of stem cells. It is also recommended that the biomechanical factors, such as mechanical stimuli, should be considered in view of bio-mimetic environments.
- 出版日期2011-8
