Assessment of Cytogenetic Damages on Human Peripheral Lymphocytes Following Gamma Rays Local Cutaneous Exposures

作者：Park Chul Song; Kim Eun Ju; Jeong Kyu Shik; Park Sang Joon; Kwak Dong Mi; Kwon Oh Deog; Rhee Man Hee; Park Seung Chun; Kim Sung Ho; Ryu Si Yoon; Kim Chun Ho; Kim Tae Hwan^*; Kang Chang Mo

来源：Pakistan Veterinary Journal, 2014, 34(1): 68-72.

• Biological dosimetry
• Human peripheral
• lymphocyte
• Partial-body irradiation
• Unstable chromosome
• aberrations

The purpose of this paper is to establish the cytogenetic analyses of human peripheral blood samples caused by simulation of partial-body exposures. Either accidental or occupational partial-body exposure to ionizing radiation poses significant health hazards that are indicated by induction of chromosome aberrations (CA). The percentages of mixtures of blood samples irradiated in vitro with 2 Gy of gamma rays were 10, 25, 50, 75 and 100.0%. Lymphocytes were cultured for 48 hr, harvested with standard procedures and then first-division metaphase cells were analyzed. It showed that the frequencies of unstable CA depend on the proportion of the irradiated blood. All frequencies of the observed CA was lower than that of predicted or calculated from 100% exposed blood, except in one case, indicating a phenomenon of %26quot;dilution%26quot; of the un-irradiated into irradiated lymphocytes that may take place a bystander effects. Our data showed that the quantification of CA in human peripheral blood lymphocytes may be an important tool of dose assessment for partial-body exposure to ionizing radiation.

• 出版日期2014