Novel glass slide preparation system for single DNA molecules analysis

作者：Zarkov Alexander; Stoynov Stoyno; Nedelcheva Veleva Marina^*

来源：Biotechnology & Biotechnological Equipment, 2014, 28(1): 112-117.

DOI：10.1080/13102818.2014.901687

• fluorescent microscopy
• single DNA molecules
• DNA stretching
• glass slide surface modification

Here we propose an easy to build up and apply glass slide preparation system for single DNA molecules stretching. It is based on fast and simple coating of a solid glass with a cocktail of acrylic monomers that are easily polymerized via ultraviolet illumination. The acrylated slides are used to successfully stretch DNA molecules in a broader pH range compared to that of the commonly used amino-silanes. Moreover, the single DNA molecules that are stretched on the acrylated slides give a brighter and more photostable signal when visualized under a fluorescent microscope.

• 出版日期2014-2