Using Pseudomonas aeruginosa PAO1 to evaluate hydrogen peroxide as a biofouling control agent in membrane treatment systems

作者：Yang Y; Kitajima M; Pham T P T; Yu L; Ling R; Gin K Y H; Reinhard M^*

来源：Letters in Applied Microbiology, 2016, 63(6): 488-494.

DOI：10.1111/lam.12674

• biocides
• biofilms
• disinfection
• membrane
• Pseudomonas

Hydrogen peroxide (H2O2) is widely used in water treatment for biofouling control and, in conjunction with catalysts, as a powerful oxidant for contaminant destruction. H2O2 could potentially serve as an antifouling agent in reverse osmosis systems in lieu of chlorine-based disinfectants. The dependence of the biocidal efficiency of H2O2 on cell density, temperature and H2O2 concentration by determining the growth, attachment and viability of the model bacterium Pseudomonas aeruginosa PAO1 was studied. For controlling growth of planktonic PAO1 cells, the minimally required H2O2 concentration depends on the cell density and temperature. The effect of H2O2 to remove the existing biofilm was found to be effective in the presence of a high concentration bicarbonate (8<bold></bold>4gl(-1)), which forms peroxymonocarbonate, a strong oxidant and disinfectant. Treatment with H2O2-bicarbonate reduced the density of live PAO1 cells, removed extracellular polymeric substances and lowered the average biofilm thickness while maintaining the integrity of the membrane, suggesting that this type of treatment may be a suitable in-place-cleaning' procedure for biofouled membranes.

• 出版日期2016-12