A Medium-Term, Rapid Rat Bioassay Model for the Detection of Carcinogenic Potential of Chemicals

作者：Tsuda Hiroyuki^*; Futakuchi Mitsuru; Fukamachi Katsumi; Shirai Tomoyuki; Imaida Katsumi; Fukushima Shoji; Tatematsu Masae; Furukawa Fumio; Tamano Seiko; Ito Nobuyuki

来源：Toxicologic Pathology, 2010, 38(1): 182-187.

DOI：10.1177/0192623309356451

• medium-term bioassay
• carcinogens
• liver GST-P
• multi-organ

The Ito Liver Model and the Ito Multi-organ Model are used in conjunction and constitute an efficient and rapid bioassay for the identification of both genotoxic and nongenotoxic carcniogenic Chemicals. The Ito Liver Model is an 8-week bioassay system that uses the number and size of foci of hepatocytes positive for glutathione S-transferase placental form (GST-P) as the end-point marker. One hundred fifty-nine compounds were tested using the Ito Liver Model: 61 of 66 hepatocarcinogens tested positive, and 10 of 43 nonliver carcinogens were also positive. The false-positive detection of noncarcinogens was lows a Single false-positive result was obtained from the 50 noncarcinogens tested. Since more than half of all known carcinogens are hepatocarcinogens in rodents, the initial 8-week bioassay is able to detect most carcinogens. The Ito Multi-organ Model is a 28-week bioassay system for the detection of carcinogens that were not identified by the Ito Liver Model. Results are evaluated by preneoplastic andneoplastic lesions in major organs. Forty-four compounds were tested using the Ito Multi-organ Model: 17 out of 17 liver carcinogens were positive. and 19 out of 22 (86%) nonliver carcinogens were positive. None of the five noncareinogens tested positive.

• 出版日期2010