Alteration in the Apoptosis Process of Rat Esophageal Epithelium with Hyperproliferation of Indigenous Bacteria under a Physiological Condition

作者：Udayanga Kankanam Gamage Sanath; Yamamoto Kyoji; Miyata Hidenori; Yokoo Yuh; Mantani Youhei; Takahara Ei ichrou; Kawano Junichi; Yokoyama Toshifumi; Hoshi Nobuhiko; Kitagawa Hiroshi^*

来源：Journal of Veterinary Medical Science, 2012, 74(5): 597-605.

DOI：10.1292/jvms.11-0516

• apoptosis
• esophageal epithelium
• immunohistochemistry
• indigenous bacteria
• rat

The apoptosis process in rat esophageal epithelium was investigated using enzyme-immunohistochemistry and transmission electron microscopy. As a result, Fas and Fas-L were expressed in the epithelial cell membrane and cytoplasm from the stratum spinosum (SS) to the stratum granulosum (SG). No TNF-R1 show immunopositivity in the cell membranes. TNF-alpha and caspase-8 were not observed in any layer. Caspase-10, cleaved caspase-3, XIAP and DNase-1 were found in the epithelial cytoplasm from the SS to the SG, whereas Bid, Apaf-1 and cleaved caspase-9 were detected only in the SG. Cytochrome c was observed as cytoplasmic granular positivity from the stratum basale (SB) and altered into homogeneous immunopositivity in the SG. Bcl-2 and Bcl-X immunopositivity was detected in cytoplasm from the SB to the SG. Immunoreactions of Bak in the cytoplasm and Bax beneath the cell membrane were observed from the upper portion of the SS with increasing intensity toward the SG. In the sites with the hyperproliferation of indigenous bacteria, TNF-R1, TNF-alpha and caspase-8 were detected in the SG and the immunopositive intensities of Bid, Apaf-1 and cleaved caspase-9 were altered to be strong. Prominently swollen cells and decreased mitochondria were ultrastructurally confirmed in the uppermost layers of stratum corneum. These findings suggest that the Fas-Fas-L-interaction initially induces apoptosis through a mitochondria-independent pathway and secondarily through a mitochondria-dependent pathway, leading to eventual epithelial cell death in the rat esophageal epithelium. The bacterial stimuli probably enhance the mitochondria-dependent pathway through the TNF-R1-TNF-alpha interaction.

• 出版日期2012-5