Acetylcholinesterase-reduced graphene oxide hybrid films for organophosphorus neurotoxin sensing via quartz crystal microbalance

作者：Tang, Shi; Ma, Wenying; Xie, Guangzhong; Su, Yuanjie^*; Jiang, Yadong

来源：Chemical Physics Letters, 2016, 660: 199-204.

DOI：10.1016/j.cplett.2016.08.025

• Acetylcholinesterase
• Reduced graphene oxide
• Biosensor
• Quartz crystal microbalance
• Organophosphorus neurotoxin

An acetylcholinesterase (AChE)-reduced graphene oxide (RGO) hybrid films based biosensor enabled by quartz crystal microbalance (QCM) has been developed for the detection of organophosphorus neurotoxin in gas phase at room temperature. To improve the sensing performance, RGO was used to immobilize large quantities of enzyme and provide a favorable microenvironment to maintain the enzyme activity. The experimental results reveal that the response of AChE-RGO/glutaraldehyde based sensors is about 8 times larger than that of the AChE with the sensitivity of 1.583 Hz/mg/m(3). 1.0 mg amount of RGO, 5% concentration of glutaraldehyde and pH 6.8 is the optimal condition of this biosensor.

• 出版日期2016-9-1
• 单位电子科技大学; 电子薄膜与集成器件国家重点实验室