Mechanisms of Ascorbyl Radical Formation in Human Platelet-Rich Plasma

作者：Shyu Kou Gi; Chang Chao Chien; Yeh Yu Chieh; Sheu Joen Rong^*; Chou Duen Suey

来源：Biomed Research International, 2014, 2014: 614506.

DOI：10.1155/2014/614506

Recently, many clinical reports have suggested that the ascorbyl free radical (Asc(center dot)) can be treated as a noninvasive, reliable, realtime marker of oxidative stress, but its generation mechanisms in human blood have rarely been discussed. In this study, we used upstream substances, enzyme inhibitors, and free radical scavengers to delineate the mechanisms of Asc . formation in human platelet- rich plasma (PRP). Our results show that the doublet signal was detected in PRP samples by using electron spin resonance, and the hyperfine splitting of the doublet signal was a(H) = 1.88 gauss and g-factor = 2.00627, which was determined to be the Asc(center dot). We observed that the inhibitors of NADPH oxidase (NOX), cyclooxygenase (COX), lipoxygenase (LOX), cytochrome P450 (CYP450), mitochondria complex III, and nitric oxide synthase (NOS), but not xanthine oxidase, diminished the intensity of the Asc . signal dose dependently. All enzyme inhibitors showed no obvious antioxidant activity during a Fenton reaction assay. In summary, the obtained data suggest that Asc(center dot) formation is associated with NOX, COX, LOX, CYP450, eNOS, and mitochondria in human PRP.

• 出版日期2014
• 单位河北医科大学