Distribution of CGRP and its receptor components CLR and RAMP1 in the rat retina

作者：Blixt Frank W; Radziwon Balicka Aneta; Edvinsson Lars; Warfvinge Karin^*

来源：Experimental Eye Research, 2017, 161: 124-131.

DOI：10.1016/j.exer.2017.06.002

• Immunohistochemistry
• Flow cytometry
• Retina
• CGRP
• CLR
• RAMP1

Calcitonin gene-related peptide (CGRP) is a 37 amino acid neuropeptide with several functions including vasodilation, the perception of painful stimuli, and inflammation. The CGRP receptor consists of two main components; calcitonin-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1). While there is a growing recognition that CGRP plays a key role in migraine, the function of CGRP in the retina has not been fully established. This study aims to investigate the distribution of CGRP and its two receptor components in the rat retina, visually by immunohistochemistry and quantitatively using flow cytometry. CGRP immunoreactivity was found in the Muller cells while CLR/RAMP1 was located in the nerve fiber layer. Furthermore, since almost all RAMP1 immunoreactive cells co-express CLR, we propose that RAMP1 expression in the retina reflects functional CGRP receptors.

• 出版日期2017-8