A study on in vitro cytotoxicity of cortisol on madin-darby kidney cells

作者：Li, Ting-Xia; Mao, Jian-Hua^*; Huang, Lei; Fu, Hai-Dong; Chen, Shuo-Hui; Liu, Ai-Min; Liang, Yu-Qin

来源：International Journal of Clinical and Experimental Medicine, 2016, 9(2): 1298-1305.

• Cortisol
• MDCK cells
• CLSM
• SRB
• caspase 3

Stress hormones play a vital role in cancer development and tumor formation. However, this mechanism is still obscure. Cortisol plays at several important biochemical roles in disease progress and development. Evaluating the dose-dependent effect of cortisol on MDCK cells is contemplated as the primary task of this experiment. Cell viability did not change significantly following cortisol exposure. Morphologically, no changes were observed in the MDCK cells. However, few cells showed an oval profile. Fluorescent and confocal analysis showed the occurrence of apoptosis significantly at 0.1 mg/L of cortisol. However, the intensity of apoptosis was significantly reduced at higher concentration (0.2 mg/L) of cortisol. The method of qPCR quantitated the mRNA expression of caspase 3, matrix metalloproteinase-9 (MMP-9) and matrix metalloproteinase-2 (MMP-2). Caspase 3 mRNA expression was significantly altered at low concentration (0.1 mg/L) while it was tremendously reduced at the higher level of cortisol (0.2 mg/L). A remarkable increase in the MRNA expression of MMP-2 and MMP-9 was executed in a dose-dependent manner. Our experimental outcome implies that cortisol may induce the apoptosis in MDCK cells.

• 出版日期2016
• 单位浙江大学