A Molecular-Level Account of the Antigenic Hantaviral Surface

作者：Li Sai; Rissanen Ilona; Zeltina Antra; Hepojoki Jussi; Raghwani Jayna; Harlos Karl; Pybus Oliver G; Huiskonen Juha T^*; Bowden Thomas A^*

来源：Cell Reports, 2016, 15(5): 959-967.

DOI：10.1016/j.celrep.2016.03.082

Hantaviruses, a geographically diverse group of zoonotic pathogens, initiate cell infection through the concerted action of Gn and Gc viral surface glycoproteins. Here, we describe the high-resolution crystal structure of the antigenic ectodomain of Gn from Puumala hantavirus (PUUV), a causative agent of hemorrhagic fever with renal syndrome. Fitting of PUUV Gn into an electron cryomicroscopy reconstruction of intact Gn-Gc spike complexes from the closely related but non-pathogenic Tula hantavirus localized Gn tetramers to the membrane-distal surface of the virion. The accuracy of the fitting was corroborated by epitope mapping and genetic analysis of available PUUV sequences. Interestingly, Gn exhibits greater non-synonymous sequence diversity than the less accessible Gc, supporting a role of the host humoral immune response in exerting selective pressure on the virus surface. The fold of PUUV Gn is likely to be widely conserved across hantaviruses.

• 出版日期2016-5-3