Aberrant histone modifications in peripheral blood mononuclear cells from patients with Henoch-Schonlein purpura

作者：Luo, Shuangyan; Liang, Gongping; Zhang, Peng; Zhao, Ming; Lu, Qianjin^*

来源：Clinical Immunology, 2013, 146(3): 165-175.

DOI：10.1016/j.clim.2012.12.009

• Henoch-Schonlein purpura
• PBMCs
• Histone modifications
• Cytokines

Henoch-Schonlein purpura (HSP), the most common type of leukocytoclastic vasculitis, is caused by T cell-mediated autoimmune reactions. In this study, we analyze histone modification patterns in peripheral blood mononuclear cells (PBMCs) of HSP patients, and investigate the expression levels of inflammatory cytokines (IFN-gamma, IL-2, IL-4, IL-6 and IL-13), transcription factors (T-bet, GATA-3 and TIM-1) and chemokines (CXCL4 and CXCL10) in HSP patients. Our results show that histone H3 acetylation and methylation are significantly enhanced in PBMCs from HSP patients. We also demonstrate specifically that marked increases in histone H3 acetylation and H3 lysine 4 trimethylation occur at the IL-4 loci in these patients. In addition, the expression levels of IL-4, IL-6, IL-13, GATA-3, TIM-1 and CXCL4 are also increased. These findings suggest that abnormal histone modifications are present in the PBMCs of patients with HSP, possibly contributing to the activation of pathological immune responses associated with HSP.

• 出版日期2013-3
• 单位中南大学