A beta-diketonate-europium(III) complex-based time-gated luminescence probe for selective visualization of peroxynitrite in living cells

作者：Wu, Jing^*; Liu, Hongjing; Yang, Yuzhu; Wang, Huan; Yang, Mei

来源：Optical Materials, 2018, 77: 170-177.

DOI：10.1016/j.optmat.2018.01.032

• Europium(III) complex
• Luminescent probe
• Peroxynitrite
• Time-gated luminescence imaging

Herein, we designed and synthesized a new beta-diketonate-Eu3+ complex-based luminescent probe for peroxynitrite (ONOO-), Eu(hdph)(3)(tpy), by using a 2,4-dimethoxyphenyl-substituted beta-diketone ligand 1,1,1,2,2,3,3-hetafluoro-6-(2,4-dimethoxyphenyl)-4,6-hexanedione (hdph) and a coligand 2,2',2 ''-terpyridine (tpy). The probe exhibits strong fluorescence with a great quantum yield of 25.6%. Upon the addition of ONOO-, the luminescence of Eu(hdph)(3)(tpy) at 607 nm was dramatically quenched due to the interaction of the 2,4-dimethoxyphenyl moiety with ONOO-, which leads to the deactivation of the excited Eu3+ complex and a 71-fold luminescence decrease. The dose-dependent luminescence decrease showed good linear relationship against the ONOO- concentration with the detection limit at nano molar concentration level. In addition, the luminescence response of Eu(hdph)(3)(tpy) to ONOO- displayed high specificity over various reactive oxygen/nitrogen species. Taking advantage of the high sensitivity and specificity, Eu(hdph)(3)(tpy) was applied for the time-gated luminescence imaging of ONOO- in HepG2 cells. The results demonstrated the practical applicability of Eu(hdph)(3)(tpy) as a luminescent probe for the long-term visualization of intracellular ONOO-.

• 出版日期2018-3
• 单位辽宁师范大学