Uncoordinated 51-like kinase 2 signaling pathway regulates epithelial-mesenchymal transition in A549 lung cancer cells

作者：Kim Young Hwan; Baek Seung Hoon; Kim Eun Kyoung; Ha Jung Min; Jin Seo Yeon; Lee Hye Sun; Ha Hong Koo; Song Sang Heon; Kim Sun Ja; Shin Hwa Kyoung; Yong Jeongsik; Kim Do Hyung; Kim Chi Dae; Bae Sun Sik^*

来源：FEBS LETTERS, 2016, 590(9): 1365-1374.

DOI：10.1002/1873-3468.12172

• autophagy
• EMT
• mTORC1

Epithelial-mesenchymal transition (EMT) is a critical response during cancer cell metastasis. In this study, we provide evidence that uncoordinated 51-like kinase 2 (ULK2) regulates EMT. Induction of autophagy by inhibition of mammalian target of rapamycin complex 1 (mTORC1) or by disruption of mTORC1 by silencing raptor significantly enhanced EMT, however, disruption of mTORC2 by silencing rictor had no effect. Knockdown of ULK2 expression significantly induced autophagy, EMT, and migration but suppressed proliferation as well as tumor growth in a xenotransplantation model, whereas silencing of ULK1 had no effect. Therefore, we suggest that ULK2 regulates EMT through modulation of autophagy.

• 出版日期2016-5