A glucose biosensor based on cytochrome c and glucose oxidase co-entrapped in chitosan- gold nanoparticles modified electrode

作者：Song, Yonghai; Liu, Hongyu; Wang, Yu; Wang, Li^*

来源：Analytical Methods, 2013, 5(16): 4165-4171.

DOI：10.1039/c3ay40399h

• LAYER-BY-LAYER
• DIRECT ELECTROCHEMISTRY
• HORSERADISH-PEROXIDASE
• BILIRUBIN OXIDASE
• CARBON NANOTUBES
• PHYSIOLOGICAL PH
• ENZYMES
• FILM
• IMMOBILIZATION
• NANOCOMPOSITE

A glucose biosensor was developed based on cytochrome c (Cyt c) and glucose oxidase (GOD) co-entrapped on gold nanoparticles (AuNPs)-chitosan (CHIT) nanocomposites which were constructed on glassy carbon electrode (GCE) by one-step electrodeposition. The surrounding Cyt c around GOD provided a biocompatible microenvironment to keep GOD's biological activity. Simultaneously, the AuNPs-CHIT nanocomposites provided a roughened surface with large surface-to-volume ratio to greatly amplify the surface coverage of GOD-Cyt c molecules and good conductivity to realize the direct electron transfer (DET) of GOD. The biosensor showed a wide detection range, low detection limit, good stability and high sensitivity. Thus, this study not only successfully achieved DET of GOD, but also constructed satisfactory point-of-care glucose monitoring.

• 出版日期2013
• 单位江西师范大学