MicroRNA319 Positively Regulates Cold Tolerance by Targeting OsPCF6 and OsTCP21 in Rice (Oryza sativa L.)

作者：Wang, Sun-ting; Sun, Xiao-li; Hoshino, Yoichiro; Yu, Yang; Jia, Bei; Sun, Zhong-wen; Sun, Ming-zhe; Duan, Xiang-bo; Zhu, Yan-ming^*

来源：PLos One, 2014, 9(3): e91357.

DOI：10.1371/journal.pone.0091357

• APOPLASTIC OXIDATIVE BURST
• PLANT MICRORNAS
• TRANSCRIPTION FACTORS
• LOW-TEMPERATURE
• GENE-REGULATION
• SMALL RNAS
• STRESS
• ARABIDOPSIS
• EXPRESSION
• LANCEOLATE

The microRNA319 (miR319) family is conserved among diverse plant species. In rice (Oryza sativa L.), the miR319 gene family is comprised of two members, Osa-miR319a and Osa-miR319b. We found that overexpressing Osa-miR319b in rice resulted in wider leaf blades and delayed development. Here, we focused on the biological function and potential molecular mechanism of the Osa-miR319b gene in response to cold stress in rice. The expression of Osa-miR319b was down-regulated by cold stress, and the overexpression of Osa-miR319b led to an enhanced tolerance to cold stress, as evidenced by higher survival rates and proline content. Also, the expression of a handful of cold stress responsive genes, such as DREB1A/B/C, DREB2A, TPP1/2, was increased in Osa-miR319b transgenic lines. Furthermore, we demonstrated the nuclear localization of the transcription factors, OsPCF6 and OsTCP21, which may be Osa-miR319b-targeted genes. We also showed that OsPCF6 and OsTCP21 expression was largely induced by cold stress, and the degree of induction was obviously repressed in plants overexpressing Osa-miR319b. As expected, the down-regulation of OsPCF6 and OsTCP21 resulted in enhanced tolerance to cold stress, partially by modifying active oxygen scavenging. Taken together, our findings suggest that Osa-miR319b plays an important role in plant response to cold stress, maybe by targeting OsPCF6 and OsTCP21.

• 出版日期2014-3-25
• 单位东北农业大学