科研之友
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摘要
It is an undeniable fact that the effect of marine toxins becomes increasingly serious to people. Paralytic shellfish poisons (PSP) are well-known sodium channel-blocking marine toxins, which block the conduction of nerve impulses and lead to a series of neurological disorders symptoms. Similarly, as inhibitors of serine/threonine protein phosphatases type 1 (PP1) and 2A (PP2A), diarrheic shellfish poisons (DSP) can destroy cytoskeleton and affect intracellular signal transduction, which causes some symptoms of gastrointestinal food allergy. Since the conventional methods to detect marine toxins had some disadvantages (e.g., complicated pretreatment, time-consuming detection, huge instruments and off-site test), bionic electronic eye (Bionic e-Eye) was designed and studied for the in-field fast measurement and real-time on-line analysis to marine toxins. Bionic e-Eye installed the homemade software-iPlate and completed the integration of image acquisition and further data processing. With the cooperation of ELISA, Bionic e-Eye used two color models-HSV and RGB to evaluate its bioanalytical performance in saxitoxin (STX) assay and okadaic acid (OA), which were the standard representations of PSP and DSP, respectively. Moreover, the microtiter plate reader (MTPR) was compared with Bionic e-Eye in the detection of marine toxins. The results demonstrated that Bionic e-Eye using saturation had the comparable precision, dynamic range, detection limit and sensitivity to MTPR in the two assays. Therefore, Bionic e-Eye has great potential to be a field-test platform and becomes the supplement of conventional off-site detection methods in measurement domain of marine toxins.
- 出版日期2017-2
- 单位浙江大学
