Potentiation of angiogenesis and regeneration by G-CSF after sciatic nerve crush injury

作者：Pan Hung Chuan; Wu Hsi Tien; Cheng Fu Chou; Chen Cheng Hsu; Sheu Meei Ling; Chen Chun Jung^*

来源：Biochemical and Biophysical Research Communications, 2009, 382(1): 177-182.

DOI：10.1016/j.bbrc.2009.03.003

• Angiogenesis
• Bone marrow
• G-CSF
• Hematopoietic stem cells

Granulocyte Colony-Stimulating factor (G-CSF) demonstrates neuroprotective effects through different mechanisms, including mobilization of bone marrow cells. However, the influence of G-CSF-mediated mobilization of bone marrow-derived cells on injured sciatic nerves remains to be elucidated. The administration of G-CSF promoted a short-term functional recovery 7 days after crush injury in sciatic nerves. A double-immunofluorescence study using green fluorescent protein-chimeric mice revealed that bone marrow-derived CD34 cells were predominantly mobilized and migrated into injured nerves after G-CSF treatment. G-CSF-mediated beneficial effects against sciatic nerve injury were associated with increased CD34 cell deposition, vascular endothelial growth factor (VEGF) expression, and vascularization/angiogenesis as well as decreased CD68 cell accumulation. However, cell differentiation and VEGF expression were not demonstrated in deposited cells. The results suggest that the promotion of short-term functional recovery in sciatic nerve crush injury by G-CSF involves a paracrine modulatory effect and a bone marrow-derived CD34 cell mobilizing effect.

• 出版日期2009-4-24
• 单位河北医科大学