Assessment of activity and mechanism of action of beta-D-glucan against dengue virus

作者：Song, Yonghong; Zhang, Wenzhi; Jaganathan, Ravindran^*

来源：Tropical Journal of Pharmaceutical Research, 2018, 17(6): 1061-1066.

DOI：10.4314/tjpr.v17i6.12

• Dengue
• beta-D-glucan
• Polysaccharide
• Antiviral
• Plaque formation
• Binding efficiency

Purpose: To assess the antiviral efficiency of beta-D-glucan (BDG) on human liver cell line (WRL68) infected with dengue virus (DENV).
Methods: Cytotoxic activity was assessed via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Solid-phase virus binding assay was used to determine the presence of a chemical affinity between dengue virus type 2 (DENV-2) and BDG. Plaque formation assay was performed to measure the suppression of DENV-2.
Results: Plaque formation assay results revealed that the inhibition of DENV infection by BDG was effective at 400 mu g/mL which occurred by inhibiting virus replication BDG inhibited DENV replication and produced minimal toxicity on WRL68 cells at 600 pg/mL in a concentration-dependent manner. Treatment of DENV-2 with the highest concentration of the BDG resulted in 60, 55, and 50 % viability at 24, 48, and 72 h, respectively. Plaque formation and binding efficiency data confirmed that BDG protected the WRL68 cells against DENV-2.
Conclusion: The results indicate that in infected cells, beta-D-glucan was found to be potent in inhibiting replication of the dengue virus.

• 出版日期2018-6
• 单位河北工程大学