Dehydrogenase-Based Reagentless Biosensors: Electrochemically Assisted Deposition of Sol-Gel Thin Films on Functionalized Carbon Nanotubes

作者：Wang Zhijie; Etienne Mathieu^*; Poeller Sascha; Schuhmann Wolfgang; Kohring Gert Wieland; Mamane Victor; Walcarius Alain

来源：Electroanalysis, 2012, 24(2): 376-385.

DOI：10.1002/elan.201100574

• Carbon nanotubes
• Electrochemically assisted deposition (EAD)
• Sol-gel
• NADH
• Dehydrogenase

Multiwalled carbon nanotubes (MWCNT) have been functionalized, for the electrocatalytic detection of NADH, by microwave treatment, electrochemical deposition of poly(methylene green) or wrapping with an Os-complex modified polymer. Sol-gel thin films have been then electrodeposited on the carbon nanotube layers for co-immobilization of D-sorbitol dehydrogenase and diaphorase when necessary and NAD+ via covalent linkage using glycidoxypropyltrimethoxysilane. The comparison of these systems shows that the electrodeposited sol-gel matrix can significantly affect the operational behavior of functionalized MWCNT. Only MWCNT wrapped with the Os-complex modified polymer and covered with a sol-gel biocomposite allowed the electrochemical detection of D-sorbitol in a reagentless configuration.

• 出版日期2012-2