Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil

作者：Lemos Tatiana Didonet^*; Figueiredo Cerqueira Aloysio de Mello; Toma Helena Keiko; da Silva Adrianna Vieira; Bartolomeu Correa Rafael Gomes; Paludo Giane Regina; Massard Carlos Luiz; Pereira Almosny Nadia Regina

来源：REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, 2012, 21(2): 137-142.

DOI：10.1590/S1984-29612012000200012

• Babesia vogeli
• Rangelia vitalii
• piroplasms
• dogs
• PCR

Rangelia vitalii is a protozoon described from dogs in the south and southeast regions of Brazil. Iris phylogenetically related to Babesia spp. that infects dogs, but data on this enigmatic parasite is still limited. The aim of this work was to detect piroplasm species in dogs in the state of Rio de Janeiro, Brazil, by 18S rRNA gene-based PCR assay, restriction fragment length polymorphism (RFLP) and sequence analyses. Of 103 dogs examined, seven (6.8%) were positive for Babesia spp. by PCR. The amplified products were digested by restriction enzymes to differentiate the Babesia species, and one sample was identified as Babesia vogeli. The pattern observed for the other six amplification products did not match with pattern described for large Babesia infecting dogs. Sequencing analysis confirmed these six samples as R. vitalii, with high homologies (99-100%) with a sequence from south Brazil. This study confirms the presence of Babesia vogeli and Rangelia vitalii circulate in domestic dogs in Teresopolis, Rio de Janeiro, Brazil.

• 出版日期2012-6