科研之友
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摘要
Growing evidence suggests that long non-coding RNAs (lncRNAs) may play critical roles in epithelial-to-mesenchymal transition (EMT) progress. However, the complex molecular mechanisms remain largely unclear. In this study, the expression levels of lncRNAs and their target genes in EMT pathway-focused was measured by lncPath (TM) microarray. Levels of representative lncRNA-metallothionein 1J, pseudogene (MT1JP) and mRNA-metallothionein 1M (MT1M) were then further verified by real-time quantitative reverse transcription PCR (qRT-PCR). The relationship of their levels with clinicopathological factors was evaluated in patients with gastric cancer (GC). Receiver operating characteristic curve was used analyses potential diagnostic values. MT1JP and MT1M were downregulated in GCs tissues from microarray analysis results, which were further confirmed by qRT-PCR. The areas under ROC curves were 0.710 and 0.657 for MT1JP and MT1M, respectively. The level of MT1JP in normal tissue is significantly higher than that in precancerous lesions and early cancers. LncRNA Microarrays provide comprehensive insights into the expressional relationship between LncRNAs and their target genes, which will be helpful for establishing rapid connections between the LncRNA regulatory mechanisms and their biological functions. The reduced expression of MT1JP and MT1M in gastric cancer tissues, its associations with tumor diameter, differentiation, lymphatic metastasis, distal metastasis, invasion and tumor-node-metastasis (TNM) stage, and its aberrant expression in early cancer and precancerous lesions suggest that they may be a potential biomarker for the diagnosis of GC.
