A novel reaction-based fluorescent probe for the detection of cysteine in milk and water samples

作者：Wang, Jialin; Wang, Hao; Hao, Yanfeng; Yang, Shaoxiang^*; Tian, Hongyu; Sun, Baoguo; Liu, Yongguo

来源：Food Chemistry, 2018, 262: 67-71.

DOI：10.1016/j.foodchem.2018.04.084

• Fluorescent probe
• Cysteine
• Sulfonate esters
• Visual sensor

A novel fluorescent probe 3'-hydroxy-3-oxo-3H-spiro [isobenzofuran-1,9'-xanthene]-6'-yl-2,4-dini-trobenzenesulfonate (probe 1) was designed and synthesized as a visual sensor for the detection of cysteine levels in milk and water samples. The addition of cysteine to the solution of probe 1 resulted in an increase in fluorescence intensity and color change, from light yellow to yellow-green. The distinct color response indicated that probe 1 could be used as a visual sensor for cysteine. Cysteine can be detected quantitatively at concentrations between 0 and 400 mu M and the detection limit of the fluorescence response to the probe was 6.5 mu M. This suggests that probe 1 could be used as a signaling tool to determine the cysteine levels in samples, such as milk and water.

• 出版日期2018-10-1
• 单位北京工商大学