科研之友
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Content The study was conducted to evaluate the effects of -linolenic acid (ALA) on frozen-thawed quality and fatty acid composition of bull sperm. For that, twenty-four ejaculates obtained from three bulls were diluted in a Tris extender containing 0 (control), 3, 5, 10 and 15ng/ml of ALA. Extended semen was incubated at 37 degrees C for 15min, to allow absorption of ALA by sperm cell membrane. The sample was chilled for 2h, packed into 0.25-ml straws and frozen in liquid nitrogen for 24h. Subsequently, straws were thawed and evaluated for total sperm motility (computer-assisted semen analysis), membrane functional integrity (hypo-osmotic swelling test), viability (eosin-nigrosin), fatty acid composition (gas chromatography) and lipid peroxidation (thiobarbituric acid-reactive substances (TBARS)). A higher (p<0.05) percentage of total sperm motility was observed in ALA groups 5ng/ml (47.74 +/- 07) and 10ng/ml (44.90 +/- 0.7) in comparison with control (34.53 +/- 3.0), 3ng/ml (34.40 +/- 2.6) and 15ng/ml (34.60 +/- 2.9). Still, the 5ng/ml ALA group presented a higher (p< 0.05) percentage of viable sperms (74.13 +/- 0.8) and sperms with intact membrane (74.46 +/- 09) than all other experimental groups. ALA concentration and lipid peroxidation in post-thawed sperm was higher in all treated groups when compared to the control group. As such, the addition of 5ng/ml of ALA to Tris extender improved quality of frozen-thawed bull spermatozoa.
- 出版日期2015-2
