Delineation of BmSXP antibody V-gene usage from a lymphatic filariasis based immune scFv antibody library

作者：Rahumatullah Anizah; Ahmad Azimah; Noordin Rahmah; Lim Theam Soon^*

来源：Molecular Immunology, 2015, 67(2): 512-523.

DOI：10.1016/j.molimm.2015.07.040

• Immune antibody library
• Lymphatic filariasis
• Phage display
• V-gene usage

Phage display technology is an important tool for antibody generation or selection. This study describes the development of a scFv library and the subsequent analysis of identified monoclonal antibodies against BmSXP, a recombinant antigen for lymphatic filariasis. The immune library was generated from blood of lymphatic filariasis infected individuals. A TA based intermediary cloning approach was used to increase cloning efficiency for the library construction process. A diverse immune scFv library of 108 was generated. Six unique monoclonal antibodies were identified from the 50 isolated clones against BmSXP. Analysis of the clones showed a bias for the IgHV3 and V kappa 1 (45.5%) and IgHV2 and V kappa 3 (27.3%) gene family. The most favored J segment for light chain is IgKJ1 (45.5%). The most favored D and J segment for heavy chain are IgHD6-13 (75%) and IgHJ3 (47.7%). The information may suggest a predisposition of certain V genes in antibody responses against lymphatic filariasis.

• 出版日期2015-10