Date seed characterisation, substrate extraction and process modelling for the production of polyhydroxybutyrate by Cupriavidus necator

作者：Yousuf R G; Winterburn J B^*

来源：Bioresource Technology, 2016, 222: 242-251.

DOI：10.1016/j.biortech.2016.09.107

• Biopolymer
• Polyhydroxybutyrate (PHB)
• Date seeds
• Process modelling
• Cupriavidus necator

Poly-3-hydroxybutrate (PHB) is a biodegradable polymer synthesised via bacterial fermentation as a means of storing carbon and energy under unbalanced growth conditions. The production cost of petroleum-based plastics is currently lower than that for biopolymers, and the carbon source is the most significant contributor to biopolymer production cost.
A feasibility study to assess the suitability of using a date seed derived media as an alternative for PHB production under various stress conditions was investigated. Results include fructose extraction from date seeds and a mass transfer model to describe the process, demonstrating that the high nutrient content of date seeds makes them a promising raw material for microbial growth and that a meaningful amount of PHB can be produced without supplementation. Maximum dry cell weight and PHB concentrations were 6.3 g/l and 4.6 g/l respectively, giving a PHB content of 73%, when an initial fructose concentration of 10.8 g/l was used.

• 出版日期2016-12