科研之友
微信
新浪微博
Facebook
分享链接
摘要
The exaggerated use of antibiotics has led to the selection of new strains of bacteria that resist to antibiotics, a situation which is found in the case of Escherichia coli strains. In this study we have evaluated the sensitivity to antibiotics of E. coli strains isolated from several types of infected wounds. This is a retrospective study on the sensitivity to antibiotics of 113 strains of E. coli isolated from infected wounds in patients hospitalized into the Emergency Hospital in Oradea between 2006-2008. Also, the objective of this study was to assess the accuracy of two tests used to determine the sensitivity to 4 selected antimicrobial drugs: the disk diffusion method and the agar dilution method. Strains of E. coli were identified based on their morpho-tinctorial, culture and especially biochemical properties, using API 20E Biomerieux tests. The antibiotic sensitivity test was performed using the disc diffusion method and the agar dilution method, prepared according to the standards of the Clinical and Laboratory Standard Institute (CLSI, 2009). The results revealed a 75% sensitivity to amikacin, 52.3% to imipenem and between 35-50% to IV-th generation cephalosporins. The lowest level of sensitivity was recorded for ampicillin (6.8%). Our attention was drawn by a lower level of sensitivity to gentamicin, that of 38.6% as compared to 70% which is found in international statistics. Sensitivity to penicillins and cephalosporins was also low, the number of resistant strains being over 50% compared to the 19.5% rate reported in the Sentry Programme in 2004. The results of disc diffusion test for E. coli and for the tested antibiotics were perfectly reproducible and comparable with the agar dilution method. In conclusion, the isolated E. coli strains are highly sensitive to the third generation aminoglycosides, fourth generation cephalosporins and carbapenem. In this study, we found the disc diffusion to be a reliable, easy and inexpensive method for testing the susceptibility of E. coli to: ampicillin, amikacin, ciprofloxacin and ceftazidime.
- 出版日期2010-10
