Development of IgY antibodies in chickens and IgG in rabbits immunized against proteins of Pythium insidiosum isolated from horses in the state of Rio de Janeiro

作者：Nunes Rangel Maria Fabiola; Lemos Luciana da Silva; Costa de Almeida Claudia Maria; Sales Lerio Gama; Vieira da Motta Olney^*

来源：Pesquisa Veterinaria Brasileira, 2010, 30(1): 87-93.

DOI：10.1590/S0100-736X2010000100014

• Pythiosis
• Pythium insidiosum
• diseases of horses
• IgY and IgG antibodies

Nunes Rangel M.F., Lemos L.S., Almeida C.M.C., Sales L.G. & Vieira-da-Motta O. 2010. Development of Polyclonal IgY antibodies in chickens and IgG in rabbits immunized against proteins of Pythium insidiosum isolated from horses in the state of Rio de Janeiro. Pesquisa Veterinaria Brasileira 30(1):87-93. Micologia Veterinaria do Setor de Doencas Infectocontagiosas, Laboratorio de Sanidade Animal do Centro de Ciencias e Tecnologias Agropecuarias, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. Alberto Lamego 2000, Horto, Campos dos Goytacazes, RJ 28013-602, Brazil. E-mail: [e-mail]
Pythiosis is caused by Pythium insidiosum and the occurrence of disease in horses was described in the North and Northwest State of Rio de Janeiro, Brazil. The disease was described in cattle, sheep, humans, and horses in different states and regions across the country. This paper describes the development of IgY and IgG polyclonal antibodies, in chicken and rabbits, respectively against proteins extracted from kunkers and hyphae of P. insidiosum from affected horses. The proteins were recognized by chicken, rabbit and horse antibodies by immunodiffusion and Western blot against majority bands of 27 and 43 KDa, and titrated by ELISA. The antibodies IgY developed by the first time against Brazilian strains of P. insidiosum may represent a valuable tool in the detection of antigens of the pathogen and contribute to further studies aimed at immunotherapy and knowledge about this disease in endemic areas in Rio de Janeiro and in Brazil.

• 出版日期2010-1