Assessment of glutathione peroxidase-1 polymorphisms, oxidative stress and DNA damage in sensitivity-related illnesses

作者：Gugliandolo Agnese; Gangemi Chiara; Calabro Carlo; Vecchio Mercurio; Di Mauro Debora; Renis Marcella; Ientile Riccardo; Curro Monica; Caccamo Daniela^*

来源：Life Sciences, 2016, 145: 27-33.

DOI：10.1016/j.lfs.2015.12.028

• Idiopathic environmental intolerances
• Impaired detoxification systems
• Erythrocyte glutathione
• Ubiquinone
• Comet assay
• Antioxidant enzyme polymorphisms

Aims: Oxidative stress increase is a key event for development of sensitivity-related illnesses (SRIs). The aim of this work was to evaluate the influence of a genetic variant in the antioxidant enzyme glutathione peroxidase (GPx1) on oxidative stress development in SRIs.
Main methods: GPx1 rs1800668 genotype, as well as glutathione, ubiquinone, and DNA damage were assessed in 34 SRI patients and 36 healthy subjects.
Key findings: Total glutathione, reduced/oxidized glutathione, and ubiquinone were significantly decreased in cases compared with controls, while DNA fragmentation was significantly increased in patients. However, these differences were not associated to GPx1 genetic background.
Significance: GPx1 rs1800668 polymorphism does not play a major role in SRI-related oxidative stress development.

• 出版日期2016-1-15