Apoptin Induces Apoptosis in Human Bladder Cancer EJ and BIU-87 Cells

作者：Zhan, Hui; Wang, Jian-Song^*; Wang, Hai-Feng; Zuo, Yi-Gang; Wang, Chun-Hui; Ding, Ming-Xia

来源：Asian Pacific Journal of Cancer Prevention, 2012, 13(1): 135-138.

DOI：10.7314/APJCP.2012.13.1.135

• Apoptin
• urinary bladder neoplasms
• apoptosis
• therapy

Objective: To investigate whether apoptin is a apoptosis-inducing protein with a potential for bladder cancer therapy. Methods: We constructed a PCDNA3/Apoptin eukaryotic expression vector, and transfected this vector into bladder cancer cell lines BIU-87 and EJ, then observed the results by RT-PCR, transmission electron microscopy, MTT assay and the flow cytometry (TUNEL method). Results: PCDNA3/Apoptin successfully induced a high level apoptosis in both bladder cancer cell lines, compared with the controls (p < 0.05). Conclusions: Apoptin can induce high level apoptosis in human bladder cancer EJ and BIU-87 cells, which suggests a potential for human bladder cancer therapy.

• 出版日期2012
• 单位昆明医科大学