科研之友
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摘要
This study is aimed to simultaneously investigate the systemic distribution, subcellular localization, and differences in the expression level between human benign and malignant tissues for LPAR1, LPAR2, and LPAR3. Immunohistochemistry (IHC) was performed for LPAR1, LPAR2, and LPAR3 on 384 formalin-fixed paraffin-embedded human tissues from 33 organs/systems in tissue microarrays. All three LPARs were found to be widely expressed in major organs/systems of humans. The IHC signals for studied LPARs were mainly localized in the nucleus and cytoplasm, but rarely seen on cellular membranes. All three LPARs have higher nuclear IHC scores in overall malignancy compared to those in benign tissues. Of the three LPARs, LPAR1 exhibited the highest expression level in both benign and malignant tissues. Liver is the only organ that exhibited significant differences in expression levels of all three LPARs in nucleus between benign hepatocytes and hepatocellular carcinoma. Seven organs (bladder, brain, colon, kidney, lung, ovary and pancreas) exhibited significant differences in IHC scores for a single LPAR (cytoplasmic or nuclear), and twenty-six organs/tissues show no significant difference in expression of LPAR1, LPAR2 or LPAR3 (cytoplasmic or nuclear) between benign and malignant tissues. It is concluded that the expression of LPARs in human organs/tissues is varied, depending on the type of organ and pathological status. This is the first study to simultaneously investigate the systemic distribution, subcellular localization and differences in expression of multiple LPARS in human benign and malignant tissues.
- 出版日期2016
- 单位重庆医科大学
