A turn-on green fluorescent thiol probe based on the 1,2-addition reaction and its application for bioimaging

作者：Huo Fangjun^*; Kang Jin; Yin Caixia; Zhang Yongbin; Chao Jianbin

来源：Sensors and Actuators B: Chemical , 2015, 207: 139-143.

DOI：10.1016/j.snb.2014.10.023

• Thiols
• 1,2-Addition
• Turn on
• Fluorescent probe
• Bioimaging

The nucleophilic nature of thiols is used to develop a novel, sensitive, and highly effective sensor. In this work, we developed a new fluorescent probe for thiols based on fluorescein and maleic anhydride. Among the tested amino acid, only GSH, Cys, Hcy, could turn on the fluorescence emission suggesting that the system was a highly selective sensor for thiols. As a typical biological thiol, GSH was used for further examining the fluorescence response of probe. It is found that probe can detect GSH quantitatively witha detection limit as low as 0.026 mu M in aqueous/DMSO solution. Moreover its potential for biological applications was confirmed by employing it for fluorescence imaging of thiol in living cells.

• 出版日期2015-2
• 单位山西大学; 中国科学院长春应用化学研究所