Application of attenuated total reflectance Fourier transform infrared spectroscopy for determination of cefixime in oral pharmaceutical formulations

作者：Kandhro Aftab A^*; Laghari Abdul Hafeez; Mahesar Sarfaraz A; Saleem Rubina; Nelofar Aisha; Khan Salman Tariq; Sherazi S T H

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2013, 115: 51-56.

DOI：10.1016/j.saa.2013.06.032

• Cefixime
• FT-IR
• Diamond cell-ATR
• PLS
• Quality control
• HPLC

A quick and reliable analytical method for the quantitative assessment of cefixime in orally administered pharmaceutical formulations is developed by using diamond cell attenuated total reflectance (ATR) Fourier transform infrared (FT-IR) spectroscopy as an easy procedure for quality control laboratories. The standards for calibration were prepared in aqueous medium ranging from 350 to 6000 mg/kg. The calibration model was developed based on partial least square (PLS) using finger print region of FT-IR spectrum in the range from 1485 to 887 cm(-1). Excellent coefficient of determination (R-2) was achieved as high as 0.99976 with root mean square error of 44.8 for calibration. The application of diamond cell (smart accessory) ATR FT-IR proves a reliable determination of cefixime in pharmaceutical formulations to assess the quality of the final product.

• 出版日期2013-11