Antioxidant activity and optimization of extraction of polysaccharide from the roots of Dipsacus asperoides

作者：Tan, Li-Hong; Zhang, Dan; Yu, Bao; Zhao, Sheng-Ping; Wang, Jian-Wei; Yao, Ling; Cao, Wei-Guo^*

来源：International Journal of Biological Macromolecules, 2015, 81: 332-339.

DOI：10.1016/j.ijbiomac.2015.08.022

• Dipsacus asperoides
• Polysaccharide
• Antioxidant activities
• Extraction optimization

Polysaccharide extraction from Dipsacus asperoides roots (DAP) was proved to possess strong antioxidant activities, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-Azobis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging activities, inhibiting 13-carotene bleaching and strong reducing power. Cell assay demonstrated that the crude DAP possessed antioxidant activity and were effective against H2O2-induced L02 cells injury. Then, response surface methodology (RSM) was applied to optimize the ultrasonic extraction of DAP. The optimum variables given by central composite design (CCD) were as follows: ratio of water to raw material, 38.61 mL/g; ultrasonic power, 308.68W; extraction time, 38.61 min; and extraction temperature, 89 degrees C. Under these conditions, the maximum yield of DAP obtained was 7.12 +/- 0.45%. Moreover, high performance liquid chromatography (HPLC) analysis suggested that the monosaccharide compositions of DAP contained primarily mannose, ribose, glucose, galactose, xylose and arabinose, with a molar ratio of 0.22:0.48:2.29:0.34:1.39:1.41. The results of the present study showed that DAP could be considered as potential sources of natural antioxidants.

• 出版日期2015-11
• 单位重庆医科大学