Analysis of the Genetic Diversity of Dendrobium candidum by ISSR, SRAP, and ScoT

作者：Jiang Ruibin; Xu Xudong; Chen Nipi; Ding Zhishan; Bo, Jin^*

来源：Journal of Biobased Materials and Bioenergy, 2017, 11(6): 590-597.

DOI：10.1166/jbmb.2017.1720

• Dendrobium candidum
• Genetic Diversity
• ISSR
• SRAP
• SCoT

Dendrobium candidum is a traditional medicinal plant critically endangered in China. Three molecular markers, namely, inter-simple sequence repeat (ISSR), sequence-related amplified polymorphism (SRAP), and start codon-targeted (SCoT), were used to analyze the genetic diversity of 40 varieties of D. candidum. Ten primers were screened for ISSR, SRAP, and SCoT PCR reactions individually, and the polymorphic ratios were 84.6%, 76.5%, and 90.3%, respectively. Analysis using the three markers showed that genetic variations were abundant among different sources of D. candidum but few among different Dendrobium species. Some cultivars also showed similarities to the wild species. Results indicated the abundant genetic diversity of D. candidum cultivars among different areas. ISSR, SRAP, and SCoT markers exhibited similar efficiencies for fingerprinting genotypes; however, SCoT was the most effective in fingerprinting D. candidum varieties.

• 出版日期2017-12
• 单位浙江中医药大学; 浙江省肿瘤医院