A Public T cell Receptor Recognized by a Monoclonal Antibody Specific for the D-J Junction of the beta-chain

作者：Frigstad T; Loset G A; Sandlie I; Bogen B^*

来源：Scandinavian Journal of Immunology, 2013, 78(4): 345-351.

DOI：10.1111/sji.12098

Abstract
It is becoming increasingly clear that T cell responses against many antigens are dominated by public alpha/beta T cell receptors (TCRs) with restricted heterogeneity. Because expression of public TCRs may be related to resistance, or predisposition to diseases, it is relevant to measure their frequencies. Although staining with tetrameric peptide/major histocompatibility complex (pMHC) molecules gives information about specificity, it does not give information about the TCR composition of the individual T cells that stain. Moreover, next-generation sequencing of TCR does not yield information on pairing of alpha- and beta-chains in single T cells. In an effort to overcome these limitations, we have here investigated the possibility of raising a monoclonal antibody (moAb) that recognizes a public TCR. As a model system, we have used T cells responding to the 91-101 CDR3 peptide of an Ig L-chain (lambda 2(315)), presented by the MHC class II molecule I-E-d. The CD4(+) T cell responses against this pMHC are dominated by a receptor composed of V alpha 3J alpha 1;V beta 6D beta J beta 1.1. Even the V(D)J junctions are to a large extent shared between T cell clones derived from different BALB/c mice. We here describe a murine moAb (AB10) of B10.D2 origin that recognizes this public TCR, while binding to peripheral T cells is negligible. Binding of the moAb is abrogated by introduction of two Gly residues in the D-J junction of the CDR3 of the beta-chain. A model for the public TCR determinant is presented.

• 出版日期2013-10