Differential binding of the HIV-1 envelope to phosphatidylserine receptors

作者：Gu Linlin; Sims Brian; Krendelchtchikov Alexandre; Tabengwa Edlue; Matthews Qiana L^*

来源：Biochimica et Biophysica Acta-Biomembranes, 2017, 1859(10): 1962-1966.

DOI：10.1016/j.bbamem.2017.06.007

• Human immunodeficiency virus
• Phosphatidylserine
• T-cell immunoglobulin mucin
• Advanced glycosylation end product-specific receptor
• Surface plasmon resonance
• Glycoproteins

Prior work has shown that the HIV-1 envelope of the human immunodeficiency virus (HIV) interacts directly with T-cell immunoglobulin mucin (TIM) family proteins. Herein, we demonstrate that HIV-1 envelope glycoproteins from varying HIV-1 clades bind differentially to TIM proteins and functionally similar proteins acting as phosphatidylserine (PtdSer) receptors. Using enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) technology, we show that lysate containing HIV-1 envelope and recombinant HIV-1 envelope glycoproteins bind TIM-4 and advanced glycosylation end product-specific receptor (AGER). The complex binding of HIV-1 UG21 gp140 to TIM-4 or AGER suggests a biphasic interaction with these proteins.

• 出版日期2017-10