Neuropeptide Y protects cerebral cortical neurons by regulating microglial immune function

作者：Li, Qijun; Dong, Changzheng; Li, Wenling; Bu, Wei; Wu, Jiang; Zhao, Wenqing^*

来源：Neural Regeneration Research, 2014, 9(9): 959-967.

DOI：10.4103/1673-5374.133140

• nerve regeneration
• microglia
• immunological activity
• neuropeptide Y
• interleukin-1 beta
• tumor necrosis factor-alpha
• I-NMDA
• neural regeneration

Neuropeptide Y has been shown to inhibit the immunological activity of reactive microglia in the rat cerebral cortex, to reduce N-methyl-D-aspartate current (I-NMDA) in cortical neurons, and protect neurons. In this study, after primary cultured microglia from the cerebral cortex of rats were treated with lipopolysaccharide, interleukin-1 beta and tumor necrosis factor-a levels in the cell culture medium increased, and mRNA expression of these cytokines also increased. After primary cultured cortical neurons were incubated with the lipopolysaccharide-treated microglial conditioned medium, peak I-NMDA in neurons increased. These effects of lipopolysaccharide were suppressed by neuropeptide Y. After addition of the neuropeptide YY1 receptor antagonist BIBP3226, the effects of neuropeptide Y completely disappeared. These results suggest that neuropeptide Y prevents excessive production of interleuldn-1 beta and tumor necrosis factor-alpha by inhibiting microglial reactivity. This reduces I-NMDA in rat cortical neurons, preventing excitotoxicity, thereby protecting neurons.

• 出版日期2014-5
• 单位河北医科大学; 河北省人民医院