Bisphenol A Disrupts Transcription and Decreases Viability in Aging Vascular Endothelial Cells

作者：Ribeiro Varandas Edna; Sofia Pereira H; Monteiro Sara; Neves Elsa; Brito Luisa; Ferreira Ricardo Boavida; Viegas Wanda; Delgado Margarida^*

来源：International Journal of Molecular Sciences, 2014, 15(9): 15791-15805.

DOI：10.3390/ijms150915791

• bisphenol A (BPA)
• HT29
• HUVEC
• LINE 1 transcription
• cellular aging

Bisphenol A (BPA) is a widely utilized endocrine disruptor capable of mimicking endogenous hormones, employed in the manufacture of numerous consumer products, thereby interfering with physiological cellular functions. Recent research has shown that BPA alters epigenetic cellular mechanisms in mammals and may be correlated to enhanced cellular senescence. Here, the effects of BPA at 10 ng/mL and 1 mu g/mL, concentrations found in human samples, were analyzed on HT29 human colon adenocarcinona cell line and Human Umbilical Vein Endothelial Cells (HUVEC). Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) transcriptional analysis of the Long Interspersed Element-1 (LINE-1) retroelement showed that BPA induces global transcription deregulation in both cell lines, although with more pronounced effects in HUVEC cells. Whereas there was an increase in global transcription in HT29 exclusively after 24 h of exposure, this chemical had prolonged effects on HUVEC. Immunoblotting revealed that this was not accompanied by alterations in the overall content of H3K9me2 and H3K4me3 epigenetic marks. Importantly, cell viability assays and transcriptional analysis indicated that prolonged BPA exposure affects aging processes in senescent HUVEC. To our knowledge this is the first report that BPA interferes with senescence in primary vascular endothelial cells, therefore, suggesting its association to the etiology of age-related human pathologies, such as atherosclerosis.

• 出版日期2014-9