科研之友
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摘要
Sleep deprivation (SD) seriously affects animal learning and memory ability. The appropriate intensity of exercise training can alleviate SD damage on brain function. This study observed the impact of different athletic intensities on working memory and BDNF protein expression in prefrontal cortex of SD rat to investigate the effect of exercise intervention on SD rats and potential mechanism. Male Sprague-Dawley rats were randomly divided into five groups, including group A as control, group B as SD model, and group C, D, and E as low, medium, and high intensity of exercise on SD model. The rats in group C, D, and E received platform exercise for 2, 4, and 8 weeks, respectively. The SD rat model was established using flower pot method. Y maze was applied to assess the ability of learning and memory. Colorimetric method was adopted to determine serum superoxide disproportionation enzyme (SOD), malondialdehyde (MDA), and cerebral cholinesterase alkali (AchE) and 5-HT levels. Western blot was selected to detect BDNF protein expression in prefrontal cortex. The correct response rate of Y maze test, serum SOD, and BDNF protein expression were reduced, while the electric shock time, serum MDA, cerebral AchE and 5-HT, and BDNF protein expression were increased in group B compared with group A (P < 0.05). Compared with group B, Y maze performance, serum SOD and MDA content, cerebral AchE and 5-HT levels, and BDNF protein expression were obviously improved in group C, D, and E. The moderate intensity exercise group exhibited the strongest effect (P < 0.05). SD rat demonstrated learning and memory ability slow down. Different intensities of exercise can improve the learning, memory, and brain function of SD rat. Moderate intensity exercise exhibited the strongest impact, which may be related to the upregulation of BDNF protein and regulation of central neurotransmitter.
